Tin oxide quantum dot based DNA sensor for pathogen detection.
Identifieur interne : 000310 ( Main/Exploration ); précédent : 000309; suivant : 000311Tin oxide quantum dot based DNA sensor for pathogen detection.
Auteurs : RBID : pubmed:23755572English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA, Bacterial.
- chemical , chemistry : Tin Compounds.
- chemical : DNA Probes.
- genetics : Vibrio cholerae.
- isolation & purification : Vibrio cholerae.
- pathogenicity : Vibrio cholerae.
- Base Sequence, Microscopy, Atomic Force, Quantum Dots, Spectroscopy, Fourier Transform Infrared, Virulence.
Abstract
We report the application of nano crystalline tin oxide quantum dots (SnO2-QDs) for electrochemical detection of Vibrio cholerae based on DNA hybridization technique. SnO2-QDs (- 1-5 nm) have been synthesized by laser ablation technique in liquid (LAL) and electrophoretically deposited onto hydrolyzed surface of indium tin oxide (ITO) coated glass electrode. A single stranded oligonucleotide probe (23 bases) have been designed form the virulent gene sequence of V. cholerae and has been immobilized onto SnO2-QDs/ITO surface for the fabrication of ssDNA/SnO2-QDs/ITO bioelectrode and these bioelectrode have been further used for DNA hybridization (dsDNA/SnO2-QDs/ITO). The electrochemical response studies have been carried out with different concentration genomic DNA (100-500 ng/microL), which indicated that SnO2 provides an effective surface to bind with the phosphate group of DNA, thus resulting in an enhanced electron transport. The hybridized electrode exhibits linear response with regression coefficient (R) 0.974, high sensitivity 35.20 nA/ng/cm2, low detection limit (31.5 ng/microL), faster response time (3 s) and high stability of 0-120 days when stored under refrigerated conditions.
PubMed: 23755572
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Tin oxide quantum dot based DNA sensor for pathogen detection.</title><author><name sortKey="Patel, Manoj K" uniqKey="Patel M">Manoj K Patel</name><affiliation wicri:level="1"><nlm:affiliation>National Physical Laboratory (CSIR), Department of Science and Technology, Centre on Biomolecular Electronics, Biomedical Instrumentation Section, Dr. K. S. Krishnan Marg, New Delhi 110012, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>National Physical Laboratory (CSIR), Department of Science and Technology, Centre on Biomolecular Electronics, Biomedical Instrumentation Section, Dr. K. S. Krishnan Marg, New Delhi 110012</wicri:regionArea></affiliation></author><author><name sortKey="Singh, Jay" uniqKey="Singh J">Jay Singh</name></author><author><name sortKey="Singh, Manish K" uniqKey="Singh M">Manish K Singh</name></author><author><name sortKey="Agrawal, Ved Varun" uniqKey="Agrawal V">Ved Varun Agrawal</name></author><author><name sortKey="Ansari, S G" uniqKey="Ansari S">S G Ansari</name></author><author><name sortKey="Malhotra, B D" uniqKey="Malhotra B">B D Malhotra</name></author></titleStmt><publicationStmt><date when="2013">2013</date><idno type="RBID">pubmed:23755572</idno><idno type="pmid">23755572</idno><idno type="wicri:Area/Main/Corpus">000586</idno><idno type="wicri:Area/Main/Curation">000586</idno><idno type="wicri:Area/Main/Exploration">000310</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>DNA Probes</term><term>DNA, Bacterial (analysis)</term><term>Microscopy, Atomic Force</term><term>Quantum Dots</term><term>Spectroscopy, Fourier Transform Infrared</term><term>Tin Compounds (chemistry)</term><term>Vibrio cholerae (genetics)</term><term>Vibrio cholerae (isolation & purification)</term><term>Vibrio cholerae (pathogenicity)</term><term>Virulence</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Tin Compounds</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Probes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Vibrio cholerae</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Vibrio cholerae</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Vibrio cholerae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Microscopy, Atomic Force</term><term>Quantum Dots</term><term>Spectroscopy, Fourier Transform Infrared</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We report the application of nano crystalline tin oxide quantum dots (SnO2-QDs) for electrochemical detection of Vibrio cholerae based on DNA hybridization technique. SnO2-QDs (- 1-5 nm) have been synthesized by laser ablation technique in liquid (LAL) and electrophoretically deposited onto hydrolyzed surface of indium tin oxide (ITO) coated glass electrode. A single stranded oligonucleotide probe (23 bases) have been designed form the virulent gene sequence of V. cholerae and has been immobilized onto SnO2-QDs/ITO surface for the fabrication of ssDNA/SnO2-QDs/ITO bioelectrode and these bioelectrode have been further used for DNA hybridization (dsDNA/SnO2-QDs/ITO). The electrochemical response studies have been carried out with different concentration genomic DNA (100-500 ng/microL), which indicated that SnO2 provides an effective surface to bind with the phosphate group of DNA, thus resulting in an enhanced electron transport. The hybridized electrode exhibits linear response with regression coefficient (R) 0.974, high sensitivity 35.20 nA/ng/cm2, low detection limit (31.5 ng/microL), faster response time (3 s) and high stability of 0-120 days when stored under refrigerated conditions.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">23755572</PMID><DateCreated><Year>2013</Year><Month>06</Month><Day>12</Day></DateCreated><DateCompleted><Year>2013</Year><Month>07</Month><Day>01</Day></DateCompleted><Article PubModel="Print"><Journal><ISSN IssnType="Print">1533-4880</ISSN><JournalIssue CitedMedium="Print"><Volume>13</Volume><Issue>3</Issue><PubDate><Year>2013</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Journal of nanoscience and nanotechnology</Title><ISOAbbreviation>J Nanosci Nanotechnol</ISOAbbreviation></Journal><ArticleTitle>Tin oxide quantum dot based DNA sensor for pathogen detection.</ArticleTitle><Pagination><MedlinePgn>1671-8</MedlinePgn></Pagination><Abstract><AbstractText>We report the application of nano crystalline tin oxide quantum dots (SnO2-QDs) for electrochemical detection of Vibrio cholerae based on DNA hybridization technique. SnO2-QDs (- 1-5 nm) have been synthesized by laser ablation technique in liquid (LAL) and electrophoretically deposited onto hydrolyzed surface of indium tin oxide (ITO) coated glass electrode. A single stranded oligonucleotide probe (23 bases) have been designed form the virulent gene sequence of V. cholerae and has been immobilized onto SnO2-QDs/ITO surface for the fabrication of ssDNA/SnO2-QDs/ITO bioelectrode and these bioelectrode have been further used for DNA hybridization (dsDNA/SnO2-QDs/ITO). The electrochemical response studies have been carried out with different concentration genomic DNA (100-500 ng/microL), which indicated that SnO2 provides an effective surface to bind with the phosphate group of DNA, thus resulting in an enhanced electron transport. The hybridized electrode exhibits linear response with regression coefficient (R) 0.974, high sensitivity 35.20 nA/ng/cm2, low detection limit (31.5 ng/microL), faster response time (3 s) and high stability of 0-120 days when stored under refrigerated conditions.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Patel</LastName><ForeName>Manoj K</ForeName><Initials>MK</Initials><Affiliation>National Physical Laboratory (CSIR), Department of Science and Technology, Centre on Biomolecular Electronics, Biomedical Instrumentation Section, Dr. K. S. Krishnan Marg, New Delhi 110012, India.</Affiliation></Author><Author ValidYN="Y"><LastName>Singh</LastName><ForeName>Jay</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Singh</LastName><ForeName>Manish K</ForeName><Initials>MK</Initials></Author><Author ValidYN="Y"><LastName>Agrawal</LastName><ForeName>Ved Varun</ForeName><Initials>VV</Initials></Author><Author ValidYN="Y"><LastName>Ansari</LastName><ForeName>S G</ForeName><Initials>SG</Initials></Author><Author ValidYN="Y"><LastName>Malhotra</LastName><ForeName>B D</ForeName><Initials>BD</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Nanosci Nanotechnol</MedlineTA><NlmUniqueID>101088195</NlmUniqueID><ISSNLinking>1533-4880</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>DNA Probes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Tin Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>18282-10-5</RegistryNumber><NameOfSubstance>stannic oxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">DNA Probes</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Microscopy, Atomic Force</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Quantum Dots</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Spectroscopy, Fourier Transform Infrared</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tin Compounds</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Vibrio cholerae</DescriptorName><QualifierName MajorTopicYN="N">genetics</QualifierName><QualifierName MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Virulence</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>6</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>6</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>7</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23755572</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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